Influence of Vitamin D Status and IL-10 Gene Promoter Polymorphism (rs1800871) on Plasma IL-10 Levels in Apparently Healthy Individuals from Southern India: A Cross-sectional Study
Published: May 1, 2023 | DOI: https://doi.org/10.7860/JCDR/2023/59455.17981
Alphienes Stanley Xavier, Alladi Charanraj Goud, Saibal Das, Sapan Kumar Behera, Sandhiya Selvarajan
1. Assistant Professor, Department of Pharmacology, AIIMS, Madurai, Tamil Nadu, India.
2. Senior Research Fellow, Department of Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.
3. Scientist D (Medical), Department of Centre for Ageing and Mental Health, ICMR, Kolkatta, West Bengal, India.
4. Senior Resident, Department of Clinical Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.
5. Additional Professor and Head, Department of Clinical Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.
Correspondence
Dr. Alphienes Stanley Xavier,
Assistant Professor, Department of Pharmacology, AIIMS, Madurai, Temporary Campus at Government Ramanathapuram Medical College, Ramanathapuram-623503, Tamil Nadu, India.
E-mail: alphclinpharm@sriramachandra.edu.in; alphclinpharm@gmail.com
Introduction: Interleukin-10 (IL-10) is a major anti-inflammatory cytokine, that plays an important role in regulating inflammatory responses of the immune system. Changes in IL-10 level and its function can cause an imbalance in immune response, which can be associated with various disease conditions.
Aim: To study the influence of vitamin D status and IL-10 gene promoter polymorphism (rs1800871) on circulating IL-10 cytokine levels in apparently healthy South Indian population.
Materials and Methods: A cross-sectional study was conducted at the Department of Clinical Pharmacology, JIPMER, Puducherry, India from January 2016 to December 2017. Apparently healthy South Indian volunteers (N=101) of either sex, age more than 18 years, were recruited for the study, after obtaining written informed consent. Serum 25-hydroxy vitamin D and plasma IL-10 levels were measured by using Chemiluminescence and Enzyme Linked Immunosorbent Assay (ELISA), respectively. IL-10 rs1800871 genotyping was performed by Real Time-polymerase Chain Reaction (RT-PCR). Values were expressed as median, Inter-quartile Range (IQR) and proportions were described as number with percentage.
Results: The median serum vitamin D and plasma IL-10 levels observed among the study population were 18.21 ng/mL IQR, 11.3-23.08 ng/mL) and 9.04pg/mL (IQR, 7.75, 11.34 pg/mL) respectively. The genotype and allele frequencies of rs1800871 were consistent with those of the African, South Asian population of 1000 genome project. Plasma IL-10 levels were not significantly different across genotypes (p-value=0.091), even though the median level among homozygous mutant (TT) volunteers was observed to be less (8.35 pg/mL vs 9.69 pg/mL, 9.83 pg/mL). The correlation between vitamin D and IL-10 levels was observed to be insignificant (p-value=0.143).
Conclusion: The present study has reported the rs1800871 genotype frequency, circulating serum vitamin D levels and plasma IL-10 levels in the apparently healthy South Indian population. IL-10 cytokine levels were not significant across the different genotype and vitamin D status groups. No significant correlation was observed between the IL-10 and vitamin D levels among the sample studied.
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